EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.4.22.2 | acetonitrile | papain retains almost all its catalytic activity after 24 h of incubation in the presence of 99% (v/v) acetonitrile with a more compact conformation | Carica papaya |
3.4.22.2 | dimethyl formamide | papain shows a complete inactivation after 24 h when exposed to those media containing 90% (v/v) dimethyl formamide because of an irreversible conformational change | Carica papaya |
3.4.22.2 | Methanol | papain shows 80% loss of activity after 24 h incubation in 90% (v/v) methanol although no global conformational change and minor secondary structure rearrangements are detected | Carica papaya |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.2 | Carica papaya | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.2 | - |
Carica papaya |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.2 | latex | - |
Carica papaya | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.2 | Bovine serum albumin + H2O | - |
Carica papaya | ? | - |
? | |
3.4.22.2 | casein + H2O | - |
Carica papaya | ? | - |
? | |
3.4.22.2 | DL-4-hydroxyphenylglycine methyl ester + H2O | asymmetric hydrolysis | Carica papaya | ? | - |
? | |
3.4.22.2 | L-Arg-7-amido-4-methylcoumarin + H2O | - |
Carica papaya | L-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.2 | additional information | papain is also able to synthesize L-aminoacylantipyrine amides, Z-Gly-Phe-NH2 and Boc-Gly-Phe-OMe, and performs hydrogenation of methyl 2-acetamidoacrylate | Carica papaya | ? | - |
? | |
3.4.22.2 | Nalpha-benzoyl-L-Arg ethyl ester | - |
Carica papaya | ? | - |
? | |
3.4.22.2 | ovalbumin + H2O | - |
Carica papaya | ? | - |
? |